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The good news is that B12 is one of the safest vitamins, but there are a few things to know about high-dose supplements and when theyre actually necessary

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The bottom line on human data: promising signals, no randomized controlled trials, and no long-term safety data in humans

Adult Dosing Dosage may vary based on different medical indications: For the initial treatment of vitamin B12 deficiency, the usual dose is 1000mcg SC/IM, given once a day for 1 week, followed by 1 dose a week for 1 month

The 28-day window assumes: Refrigerated storage between 2 C and 8 C (36 F 46 F) Aseptic technique when withdrawing from the vial No visible particulate matter, cloudiness, or color change An intact rubber septum that re-seals after each puncture If any of these conditions are not met, discard the vial earlier

B12 is necessary for the production of serotonin, a chemical responsible for regulating mood